CROP-IT is a tool developed to assist biologists in designing CRISPR/CAS9 sgRNAs, by predicting the off-target sites and ranking them according to the chances of occurrence. We also provide feature to find CRISPR/CAS9 binding sites, given a genomic interval and PAM sequence of interest. You can access both the features as follows:
This leads to you the "Predict off-target sites" page, where you can input the target sgRNA sequence and get the predicted off-target sites. The results are mailed to the email-ID provided by the user.
This leads to you the "Identify CRISPR/CAS9 sites" page, where you can input the genomic interval of interest (Max. size = 250bp) and the tool outputs a list of all possible sgRNAs for a given PAM sequence. User can find potential target sequences for both Single sgRNA (ranked) and Double Nickase systems. The results are mailed to the email-ID provided by the user.